Validation of the Analytical Method for the Determination of Flavonoids in Broccoli

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Validation of the Analytical Method for the Determination of Flavonoids in Broccoli

A simple, accurate and selective HPLC method was developed and validated for determination of quercetin and kaempferol, which are the main flavonols in broccoli. The separation was achieved on a reversed-phase C18 column using a mobile phase composed of methanol/water (60/40) and phosphoric acid 0.2% at a flow rate of 1.0 ml·min. The detection was carried out on a DAD detector at 370 nm. This m...

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ژورنال

عنوان ژورنال: Journal of Horticultural Research

سال: 2014

ISSN: 2300-5009

DOI: 10.2478/johr-2014-0016